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  Chemistry and Biotechnology - Fluid Imaging Technologies - FlowCam 8000



FlowCam 8000
FlowCam® technology combines the benefits of digital imaging, flow cytometry, and microscopy into a single instrument to help researchers quickly and easily measure and count microscopic particles in a fluid medium. FlowCam automatically analyzes thousands of particles per minute, ranging in size from 1µm – 6mm.
VisualSpreadsheet, partner software to the FlowCam, is an interactve, image-based analytcal sofware that generates 42 particle measurements per cell. Filter, sort, and classify data based on user-defined criteria. Create libraries to automate classification for future sample analyses.
Main Features:
icon 1. Visual Spreadsheet™ software generates 40+morphological measurements, supporting semiautomated identification and classification of plankton and other particles
2. Larger field of view and faster camera increases data throughput
3. Use of high powered laser and more sensitive PMTs, along with redesign of optical path, enhances fluorescence signal analysis
4. Integrated auto-focus mechanism simplifies focusing, and provides consistent, highresolution images
5. Snap-In’ flow cell holder reduces time required to change magnifications
6. Automated self-rinse and self-cleaning cycles reduce cross contamination and increase life of flow cells
Standard Certification:
icon USP <1788>
Application:
icon 1. Particle analysis(aggregation, morphology, particle size, quantity)
2. Science and technology industry (semiconductor, LED, optoelectronics, etc.)
3. Protein treatment, pharmaceutical related industries
4. Algae research
5. Automatic algae classification and counting
6. Zooplankton Research
7. Chemical particle research
8. Quality Control (drug, food)
9. Drinking water safety
10. Sewage treatment
Specification:
Introduction video:
How the FlowCAM imaging particle analysis system works
https://www.youtube.com/watch?v=gqob_lOT19c

FlowCam 8000 Aquatic

Particle Size Range

3 μm to 600 μm

Magnification & Flow Cells

20X (~200X magnification), flow cell depth option: 50 μm Field-of-View (FOV)

10X (~100X magnification), flow cell depth option: 100 μm FOV

4X (~40X magnification), flow cell depth options: 300 μm and 600 μm FOV

Sample Processing Capability

0.05 mL/minute at 20X and up to 3 mL/minute at 4X

Measured Parameters

Basic Shape Parameters: Area, Aspect Ratio (width/length), Area Based Diameter (ABD), Equivalent Spherical Diameter (ESD), Length, Volume (ABD-based), Volume (ESD-based), Width, 3 Biovolume Measurements

Advanced Morphology Parameters: Area (Filled), Circle Fit, Circularity, Circularity (Hu), Compactness, Convex Perimeter, Convexity, Elongation, Fiber Curl, Fiber Straightness, Geodesic Aspect Ratio, Geodesic Length, Geodesic Thickness, Perimeter, Roughness, Symmetry

Fluorescence Detection & Measurements: Channel 1 Area, Channel 1 Peak, Channel 1 Width, Channel 1 Area, Channel 1 Peak, Channel 1 Width, Channel 2/Channel 1 Ratio

Gray Scale and Color Measurements: Average Blue, Average Green, Average Red, Edge Gradient, Intensity, Blue/Green Ratio, Red/Blue Ratio, Red/Green Ratio, Edge Gradient, Intensity, Sigma Intensity, Sum Intensity, Transparency

Camera

High resolution (1920x1200 pixels) CMOS. Monochrome and color available.

Frame Rate

Shutters up to 100 frames per second.

Fluidics

Micro syringe pump with multiple sizes to optimize flow rates: 0.5 mL, 1 mL, 5 mL

Data Acquisition Method

FlowCam 8400 - fluorescence based laser triggering and auto imaging

FlowCam 8100 - auto imaging

Fluorescence Emission & Detection

Excitation Options (488 nm, 532 nm, 633 nm) with 2-Channel Fluorescence Detection:

- 488 nm laser - Ch 1: 650nm long pass / Ch 2: 525nm ± 15nm (FITC)

- 532 nm laser - Ch 1: 650 long pass / Ch 2: 575nm ± 30nm (Phycoerythrine)

- 633 nm laser- Ch 1: 700nm ± 10nm (Chlorophyll) / Ch 2: 650nm ± 10nm (Phycocyanin)

 

FlowCam 8000 Biopharm

Particle Size Range

1 μm to 100 μm

Magnification & Flow Cells

20X (~200X magnification), flow cell depth option: 50 μm Field-of-View (FOV)

10X (~100X magnification), flow cell depth option: 100 μm FOV

4X (~40X magnification), flow cell depth options: 300 μm and 600 μm FOV

Measured Parameters

Basic Shape Parameters: Area, Aspect Ratio (width/length), Area Based Diameter (ABD), Equivalent Spherical Diameter (ESD), Length, Volume (ABD-based), Volume (ESD-based), Width, 3 Biovolume Measurements

Advanced Morphology Parameters: Area (Filled), Circle Fit, Circularity, Circularity (Hu), Compactness, Convex Perimeter, Convexity, Elongation, Fiber Curl, Fiber Straightness, Geodesic Aspect Ratio, Geodesic Length, Geodesic Thickness, Perimeter, Roughness, Symmetry

Fluorescence Detection & Measurements: Channel 1 Area, Channel 1 Peak, Channel 1 Width, Channel 1 Area, Channel 1 Peak, Channel 1 Width, Channel 2/Channel 1 Ratio

Gray Scale and Color Measurements: Average Blue, Average Green, Average Red, Edge Gradient, Intensity, Blue/Green Ratio, Red/Blue Ratio, Red/Green Ratio, Edge Gradient, Intensity, Sigma Intensity, Sum Intensity, Transparency

Camera

High resolution (1920x1200 pixels) CMOS. Monochrome and color available.

Frame Rate

Shutters up to 100 frames per second.

Fluidics

Micro syringe pump with multiple sizes to optimize flow rates: 0.5 mL, 1 mL, 5 mL

Optional Fluorescence Emission & Detection

Excitation Options (488 nm, 532 nm, 633 nm) with 2-Channel Fluorescence Detection:

- 488 nm laser - Ch 1: 650nm long pass / Ch 2: 525nm ± 15nm

- 532 nm laser - Ch 1: 650 long pass / Ch 2: 575nm ± 30nm

- 633 nm laser- Ch 1: 700nm ± 10nm / Ch 2: 650nm ± 10nm

VisualSpreadsheet® Software

Interactive, image-based analytical software that generates 40+ particle measurements per cell. Filter, sort, and classify data based on user-defined criteria. Create libraries to automate classification for future sample analyses.

 

 
 
 
 
 
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