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  Chemistry and Biotechnology - ThermoFisher - High Performance Liquid Chromatography (HPLC)
Ultimate High Performance Liquid Chromatography System (UHPLC)

Liquid chromatography (LC)
More versatile UHPLC for all LC Systems
UHPLC delivers important benefits – faster runs, better resolution, and lower operating costs. The extended performance of the Thermo Scientific™ Dionex™ UltiMate™ 3000 systems offer UHPLC compatibility across all modules, ensuring maximum performance.
UHPLC compatibility has been added to all standard and basic automated Dionex HPLC systems, and we’ve extended the UHPLC performance of the UltiMate 3000 RSLC systems, giving you more versatility for your analytical needs.
The UltiMate 3000 HPLC systems offer UHPLC compatibility across all modules, ensuring maximum performance. With flow rates from 20 nL/min to 10 mL/min and a wide range of pumping, sampling, and complementary detection modules, the UltiMate 3000 series provides solutions for all your chromatography needs.
Features and Benefits:
• UHPLC capabilities for all RS and standard SD systems
• 620 bar (9000 psi) maximum pressure for basic and standard SD systems
• x2 Dual gradient systems deliver a unique platform for workflow automation for boosting productivity and separation results
• Systems are controlled using Thermo Scientific™ Dionex™ Chromeleon™ Chromatography Data System software – providing Intelligent Functionality and Operational Simplicity™
• Systems are offered with Thermo Scientific™ Dionex™ Viper™ and nanoViper™ Fingertight fitting systems – virtually, zero-dead-volume connections even at UHPLC pressures
UltiMate 3000 XRS LC Systems
UltiMate 3000 XRS LC Systems
The UltiMate 3000 XRS LC system is the new benchmark for high end UHPLC separation and detection technologies. It is designed for ultra-high resolution or very fast chromatography while providing perfect LC/MS integration features and pressures up to 1250 bar (18,125 psi).
• More quality data with fewer experiments
• Better results for the most demanding complex samples
• Extreme low gradient delay volume
• High sample capacity of up to 12 well plates for high throughput screening
• Ideally equipped for early drug development, support of clinical studies and life science research
UltiMate 3000 Rapid Separation LC Systems
UltiMate 3000 Rapid Separation LC Systems
The UltiMate 3000 RS systems integrate unique hardware features for ultrafast separations and excellent resolution using columns with small particles. The best choice for pharma, life science, and food & beverage laboratories demanding versatility and future-proof investment.
• Binary or quaternary systems support UHPLC and conventional HPLC applications
• Extensive flow-pressure-footprint for ultrafast, ultrahigh resolution separations – supporting also large column diameters
• Column pressures up to 1000 bar
• x2 Dual gradient configurations for ultimate productivity solutions
UltiMate 3000 Standard SD LC Systems
UltiMate 3000 Standard SD LC Systems
The UltiMate 3000 Standard SD LC systems provide the right solution for demanding, analytical, and semi-preparative LC applications.
• Optimal performance and reliability for conventional LC applications
• 620 bar (9000 psi) maximum pressure and 100 Hz detectors for UHPLC compatibility
• Widest range of system configurations for maximum application flexibility
• Flow rates up to 10 mL/min covering all application needs
• Biocompatible version available (up to 350 bar)
UltiMate 3000 Basic LC Systems
UltiMate 3000 Basic LC Systems
The UltiMate 3000 Basic systems are cost-effective solutions for conventional HPLC and UHPLC. Fully-scale able, modular basic systems are designed to provide consistent, robust operation and are even compatible with UHPLC applications.
• Cost-effective system for routine applications
• 620 bar (9000 psi) maximum pressure and 100 Hz data rate for UHPLC compatibility
• Optional Autosampler Column Compartment with integrated sample and column temperature control
Reliable Solvent Delivery
The UltiMate 3000 SD pump family offers the most complete choice in the industry and is the right solution for a wide range of applications. All models combine unrivaled flow and gradient performance with outstanding reliability and durability.
• Wide operating flow rate range up to 10 mL/min at pressures of up to 620 bar
• SmartFlow™ ensures optimal performance independent of mobile phase composition
• SpinFlow™ mixing technology perfectly balances gradient delay volume against mobile phase mixing efficiency
UltiMate 3000
The Art of Mixing
In gradient elution, complete mixing is necessary to achieve a smooth baseline for high signal-to-noise ratios, and accurate peak identification. The mixer requirements are challenging because the mixer must reliably provide highest possible performance over a wide application range with varying solvent ratios, solvent miscibilities, and flow rates. To meet these requirements, the unique SpinFlow™ mixing design was developed, using a two-step process of radial mixing followed by longitudinal mixing. The mixer can be easily adapted to fit specific method requirements, from high-speed LC/MS to high-sensitivity TFA applications.
Modular system design for ultimate versatility
Dionex LC modules integrate innovation and intelligent features into a broad selection of autosampler, injector, pump, thermostatted column compartment, and unique detector modules. We offer a wide variety of accessories and supplies for use with our LC systems, to accommodate any analytical application.
UltiMate 3000 Pumps
UltiMate 3000 Basic LC Systems
The UltiMate 3000 pump family offers the most complete choice in the industry. From conventional LC applications to UHPLC methods, the UltiMate 3000 pumps provide industry leading flow stability and precision.
The pumps are available in isocratic, binary, quaternary and unique dual gradient (x2 Dual) configurations.
Samplers and Column Compartments
Samplers and Column Compartments
The UltiMate 3000 autosamplers ensure reliable, precise, and accurate injections from nL to mL sample volumes. With extremely low carry-over and our unique in-sampler fraction collection option, advanced 2D-LC workflows are easily mastered even in routine laboratories.
Autosamplers:Split-Loop, Pulled-Loop with integrated column compartment
Fraction Collectors:Fraction Collector, Autosampler/Fraction Collector, MALDI Spotter
Column Compartments:Integrated Switching Valves
Optical Detection:The UltiMate 3000 optical detectors are available for UV-vis absorbance, fluorescence, and refractive-index detection for a wide variety of analytes.
Charged Aerosol Detection:The Thermo Scientific™ Dionex™ Corona™ Charged Aerosol detectors provide near-universal detection for non- or semi-volatile compounds. They provide consistent response independent of analyte structure.
Electrochemical Detection:The highly sensitive and analyte selective Thermo Scientific™ Dionex™s Electrochemical Detectors are used for routine analysis in complex sample matrices.
Chromeleon 7
Chromeleon 7 Chromatography Data System (CDS)
Streamline your Laboratory
Enjoy Operational Simplicity

• Increase productivity
• Control all your instrumentation
• Achieve regulatory compliance (incl. 21 CFR Part 11)
• Simplify administration
• Connect your laboratory
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